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JAYPEE JOURNALS
International Scientific Journals from Jaypee
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1.  Original Article
A Comparative Study of Lipid Profile in Obese and Nonobese Men attending Master Health Checkup
S Vinod Babu, Anusha R Jagadeesan, Jothimalar Ramalingam
[Year:2017] [Month:July-December] [Volume:21 ] [Number:2] [Pages:102] [Pages No:73-75] [No of Hits : 1065]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0024 | FREE

ABSTRACT

Introduction: Obesity is emerging as an epidemic worldwide. Obesity is associated with a number of comorbid conditions, such as diabetes mellitus, hypertension, cancer, dyslipidemia, cardiovascular abnormalities, anemia, obstructive sleep apnea, and psychosocial abnormalities.

Aim: This study aims at comparing the lipid profile levels of obese and nonobese men.

Materials and methods: This was a case-control study conducted at a tertiary care center. Totally, 80 men in the age group of 20 to 47 years attending the master health checkup were included in the study, out of which 40 men with normal body mass index (BMI) of 18 to 25 belonged to group I and 40 men with increased BMI of 30 and above belonged to group II. Lipid profile parameters, such as triglycerides (TGLs), total cholesterol, high-density lipoprotein (HDL) cholesterol, and lowdensity lipoprotein (LDL) cholesterol were estimated in them. The data were statistically analyzed using Statistical Package for the Social Sciences (SPSS) software version 15.0.

Results: Statistically significant difference was found in the total cholesterol levels with a p-value of 0.040 while the difference in LDL cholesterol was statistically highly significant with a p-value of 0.040.

Conclusion: Among lipid profile parameters, only total cholesterol and LDL cholesterol showed significant difference between the obese and nonobese individuals. However, the other parameters like HDL cholesterol and TGLs did not show any significant difference.

Keywords: Body mass index, Lipid profile parameters, Lowdensity lipoprotein cholesterol, Obese, Total cholesterol.

How to cite this article: Babu SV, Jagadeesan AR, Ramalingam J. A Comparative Study of Lipid Profile in Obese and Nonobese Men attending Master Health Checkup. Indian J Med Biochem 2017;21(2):73-75.

Source of support: Nil

Conflict of interest: None

 
2.  Original Article
Thyroid Function Status in Indian Adult Nonpregnant Females in Ranchi, India
Barun K Chakrabarty, Binay Mitra, Bhaskar Shahbabu, Nandita Hazra, Sarvinder Singh
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:25-29] [No of Hits : 802]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0014 | FREE

ABSTRACT

Aim: Thyroid disorders are one of the most common endocrine diseases in India. Thyroid disorders are more common in women than in men and contribute to significant morbidity. In this postiodization era, there is paucity of pan-Indian data of thyroid disorder status among adult nonpregnant women. This study was done to analyze the thyroid hormone levels in women of Jharkhand region, which is traditionally known to be an iodine-deficient area.

Materials and methods: Three hundred and forty nonpregnant adult females in Ranchi area who were consuming iodized salt formed part of the study group. Clinical evaluation was done by a gynecologist pertaining to thyroid illness. Thyroid function tests encompassing triiodothyronine, thyroxine, and thyroidstimulating hormone were carried out by quantitative enzyme immunoassay method. Thyroid status of the population was defined as per kit reference range.

Results: Subjects with age range 20 to 67 years were divided into three groups as per clinical status of thyroid disorder, viz. total, disease free, and control. A total of 19.6% had biochemical evidence of thyroid disorder and 82.4% were euthyroid as per reference ranges in kit literature. Out of hypothyroid subjects, 3.2% had clinical and 14.4% had subclinical hypothyroidism. In the study group, no subjects were detected to have overt or subclinical hyperthyroidism. Multiple comparison analysis was done with Statistical Package for the Social Sciences version 20.0, a statistical software package.

Discussion: This is the first study in Jharkhand area on nonpregnant adult female population that are getting iodine sufficient foods in an iodine-deficient region. The study showed high prevalence of thyroid disorders in the study group. Hypothyroidism, predominantly subclinical hypothyroidism, is prevalent among women in this region.

Keywords: Iodine sufficient, Nonpregnant adult female, Subclinical hypothyroidism, Thyroid disorders.

How to cite this article: Chakrabarty BK, Mitra B, Shahbabu B, Hazra N, Singh S. Thyroid Function Status in Indian Adult Nonpregnant Females in Ranchi, India. Indian J Med Biochem 2017;21(1):25-29.

Source of support: Nil

Conflict of interest: None

 
3.  Research Article
Comparative Study of Serum Calcium in Preeclampsia and Normal Pregnancy at Government Medical College and Hospital, Aurangabad City, India
Sunita M Aghade, Jayshree S Bavikar
[Year:2017] [Month:July-December] [Volume:21 ] [Number:2] [Pages:102] [Pages No:147-150] [No of Hits : 784]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0039 | FREE

ABSTRACT

Introduction: Preeclampsia, a pregnancy-specific disorder, is a global health problem. The major mineral calcium has been proposed to play an important role in the pathogenesis of preeclampsia. The present study was aimed to compare the level of serum calcium in normal pregnant women and in preeclampsia and determine the association of serum calcium with severity of the disease.

Study design: This study included 60 pregnant women diagnosed with preeclampsia as cases and 60 healthy pregnant women as controls in the third trimester of gestation. The concentration of total serum calcium was measured in both groups.

Results: Serum calcium level was significantly decreased in preeclamptic women than in normal pregnant women. Serum calcium showed significant negative correlation with systolic and diastolic blood pressure.

Conclusion: Hypocalcemia found in preeclamptic women in our study suggests that serum calcium may have a role in the etiopathogenesis of this disorder. Routine estimation of serum calcium may be useful as a diagnostic marker in preeclampsia.

Keywords: Hypocalcemia, Preeclampsia, Pregnancy, Serum calcium.

How to cite this article: Aghade SM, Bavikar JS. Comparative Study of Serum Calcium in Preeclampsia and Normal Pregnancy at Government Medical College and Hospital, Aurangabad City, India. Indian J Med Biochem 2017;21(2):147-150.

Source of support: Nil

Conflict of interest: None

 
4.  Original Article
Comparison of measured Serum Total Carbon Dioxide with calculated Bicarbonate calculated from Arterial Blood Gas Analysis
Thuthi Mohan, B Vinodh Kumar
[Year:2017] [Month:July-December] [Volume:21 ] [Number:2] [Pages:102] [Pages No:76-80] [No of Hits : 781]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0025 | FREE

ABSTRACT

Introduction: Calculated bicarbonate from venous sample and measured total carbon dioxide (TCO2) from arterial blood gas (ABG) have shown good agreement in some studies, while conflicting results have been obtained in few other studies. The objective of this study is to compare and assess the degree of agreement between the measured TCO2 and calculated bicarbonate and also whether they can be used interchangeably in our laboratory.

Materials and methods: We prospectively analyzed 89 ABG samples requested for calculated bicarbonate and then measured TCO2 from venous blood samples drawn simultaneously from the same participants between November 2016 and April 2017.

Results: Measured TCO2 results ranged from 5.7 to 39.9 mmol/L (mean 23.45 mmol/L), while calculated bicarbonate ranged from 9 to 40 mmol/L (mean 24.36 mmol/L). The values of TCO2 and bicarbonate correlated well (r = 0.95, p < 0.001), with the correlation given by the equation, y = 0.884x + 3.605. The bias obtained was -0.9 mmol/L and the standard deviation (SD) was 1.62 mmol/L. The limits of agreement (LOA) were -4.1 to 2.3 mmol/L, with a span of 6.4 mmol/L. Out of the 89 values, 85 (95.05%) were within the LOA.

Conclusion: In majority of the cases, the calculated bicarbonate concentration from ABG showed a good correlation to the measured venous TCO2 concentration. Despite this excellent correlation, TCO2 did not show good agreement with calculated bicarbonate when Story and Poustie’s criteria were applied, especially in cases of bicarbonate less than 20 mmol/L. Hence, clinicians should be aware of this discrepancy and be cautious when using measured TCO2 and calculated bicarbonate interchangeably in the assessment and management of acid-base disorders, especially in patients with metabolic acidosis.

Keywords: Arterial blood gas, Bicarbonate, Henderson- Hasselbalch equation, Metabolic acidosis, Total carbon dioxide.

How to cite this article: Mohan T, Kumar BV. Comparison of measured Serum Total Carbon Dioxide with calculated Bicarbonate calculated from Arterial Blood Gas Analysis. Indian J Med Biochem 2017;21(2):76-80.

Source of support: Nil

Conflict of interest: None

 
5.  Original Article
Comparison of BiliCare Transcutaneous Bilirubinometer with Standard Laboratory Assay
Jayesh Warade
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:1-4] [No of Hits : 722]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0009 | FREE

ABSTRACT

Introduction: Jaundice is the visible manifestation of chemical bilirubinemia and is characterized by yellow discoloration of skin. In a pediatric emergency department, evaluation of jaundice includes a precise medical history, physical examination, and estimation of bilirubin by laboratory tests. Measurement of total serum bilirubin (TSB) by transcutaneous bilirubin (TcB) is interpreted according to the age (in hours) of the newborn infant. Studies have also indicated that the TcB determination is more accurate than visual estimation methods.

Aim: To compare BiliCare System against standard laboratory testing for bilirubin estimation.

Results: The coefficient of correlation was r = 0.7245, and the standard deviation between the mean values of TcB and TSB is ±0.55.

Conclusion: It is concluded from the study that there is good correlation between TcB measurement and laboratory estimation of total bilirubin levels. It is recommended that transcutaneous measurement can be used for initial screening of jaundice as well as monitoring of phototherapy in neonates.

Keywords: BiliCare, Bilirubin, Jaundice, Kernicterus, Transcutaneous.

How to cite this article: Warade J. Comparison of BiliCare Transcutaneous Bilirubinometer with Standard Laboratory Assay. Indian J Med Biochem 2017;21(1):1-4.

Source of support: Nil

Conflict of interest: None

 
6.  Original Article
Vitamin D Status in Pregnancy: Fetomaternal Outcome and Correlation with Cord Blood Vitamin D
Mamta Gupta, Arijit Debnath, Sanjay Jain, Vandana Saini, Somosri Ray
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:42-48] [No of Hits : 701]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0018 | FREE

ABSTRACT

Introduction: Hypovitaminosis D in pregnancy has been reported to cause various maternal effects, i.e., hypocalcemia, subclinical myopathy, increased risk of preeclampsia (PE), gestational diabetes mellitus (GDM), cesarean sections, and fetal effects, i.e., neonatal tetany, hyperbilirubinemia congenital rickets, infantile rickets, etc. Only few Indian studies are available in this regard.

Objective: To estimate serum vitamin D levels in pregnant women, cord blood, and study fetomaternal outcomes.

Materials and methods: A prospective observational study was conducted on 54 consecutive pregnant women and their newborn babies. Serum 25-hydroxy vitamin D [25(OH)D] level was estimated in all women at the time of admission in labor ward. They were followed up to delivery and 48 hours postpartum. Vitamin D was also estimated in cord blood collected during delivery. All results were recorded and analyzed statistically.

Results: The mean 25(OH)D level in pregnancy was 6.81 ± 7.38 ng/mL. The mean 25(OH)D level in their babies (cord blood) was 6.34 ± 7.05 ng/mL. There was very strong positive correlation between maternal and fetal serum 25(OH)D levels (p-value 0.001, r-value 0.9). Vitamin D deficiency was strongly associated with obesity, PE, and GDM (p-value 0.001). Neonatal jaundice and tetany were also significantly associated with severe vitamin D deficiency.

Conclusion: Low levels of vitamin D have been observed in pregnant women and their newborn babies. Hypovitaminosis D has been associated with adverse fetomaternal outcomes. As there is a strong correlation of maternal and neonatal levels, supplementing vitamin D in a pregnant women might improve these adverse pregnancy outcomes.

Keywords: Cord blood vitamin D, Fetomaternal outcome, Hypovitaminosis D, Vitamin D status in pregnancy.

How to cite this article: Gupta M, Debnath A, Jain S, Saini V, Ray S. Vitamin D Status in Pregnancy: Fetomaternal Outcome and Correlation with Cord Blood Vitamin D. Indian J Med Biochem 2017;21(1):42-48.

Source of support: Nil

Conflict of interest: None

 
7.  Original Article
Evaluation of Serum Magnesium Level along with Lipid Profile in a Gujarati Population diagnosed with Diabetes Mellitus
Sandip S Sendhav, Amit Kakaiya, Bijoya Chatterjee
[Year:2017] [Month:July-December] [Volume:21 ] [Number:2] [Pages:102] [Pages No:112-116] [No of Hits : 658]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0032 | FREE

ABSTRACT

Aims and objectives: Diabetes is a common endocrinal disorder. Abnormal lipid and magnesium levels are observed in diabetes in many studies. The current study was done with an aim to find the relationship between lipid with magnesium and diabetes mellitus in Gujarati population.

Materials and methods: The cross-sectional study included 60 diabetics and 50 healthy subjects. Each subject was interviewed, examined, and investigated for serum lipid profile and magnesium. A 12-hour overnight fasting was recommended. The statistical analysis of data obtained was done by Student’s t-test and calculation of Pearson correlation coefficient.

Result: Routine biochemical investigations showed a significant rise (p < 0.01) of fasting plasma glucose, triglycerides (TGs), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), and very low-density lipoprotein-cholesterol (VLDL-C) in diabetics, in comparison with controls (p < 0.01). Among diabetics, males have significantly higher (p < 0.01) TC, TG, and LDL-C while significantly lower high-density lipoprotein-cholesterol (HDL-C). Significant inverse correlation of magnesium with TC (r = -0.18), TGs (r = -0.14), LDL-C (r = -0.27), fasting blood sugar (FBS; r = -0.12) and direct correlation with HDL-C (r = 026) were observed in cases.

Conclusion: The major highlights of the current study are lower magnesium levels in cases compared with controls. In the current study, serum magnesium level has been found to be inversely related to cholesterol, TG, and LDL-C levels, while it is directly associated with HDL-C level. Hence, it could play a role in controlling the risk of coronary artery disease (CAD)- associated morbidities in future.

Keywords: Coronary artery disease, Diabetes mellitus, Lipid profile, Magnesium.

How to cite this article: Sendhav SS, Kakaiya A, Chatterjee B. Evaluation of Serum Magnesium Level along with Lipid Profile in a Gujarati Population diagnosed with Diabetes Mellitus. Indian J Med Biochem 2017;21(2):112-116.

Source of Support: Nil

Conflict of interest: None

 
8.  Original Article
Prediabetes and Undiagnosed Diabetes Mellitus: The Hidden Danger.
Suprava Patel, Rachita Nanda, Jessy Abraham, Sibasish Sahoo, Anirban Ganguly, Eli Mohapatra
[Year:2017] [Month:July-December] [Volume:21 ] [Number:2] [Pages:102] [Pages No:91-95] [No of Hits : 617]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0028 | FREE

ABSTRACT

Introduction: Diabetes is considered as a major challenge to the public health system in India. Recent articles clearly mention that the hidden danger in the form of prediabetes and undiagnosed diabetes is greatly adding to the burden silently. Awareness regarding the same, particularly among youth, can help diagnose the condition very early and thus, initiate early management.

Objectives: With an aim to estimate the frequency of prediabetes and undiagnosed diabetes in the adult population, a camp was organized in our institute to screen the adults in our locality by estimating fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c).

Materials and methods: A total of 246 individuals were selected for analysis after excluding the known diabetic cases. Height, weight, pulse, blood pressure (BP), waist circumference, and body mass index (BMI) were measured. Plasma fasting sugar and fasting serum lipid profile were analyzed. The HbA1c was estimated in hyperglycemic subjects.

Results: The frequency of hyperglycemia in the study population was found to be 28%. The total frequency of prediabetes was 18.3% and that of undiagnosed diabetes was 9.75%. The raised sugar could be significantly associated with age, waist circumference, BMI, hypertriglyceridemia, and cholesterol-tohigh- density lipoprotein (Chol:HDL) ratio. Aging, greater BMI, hypertriglyceridemia, and raised low-density lipoprotein (LDL) depicted significant odds ratio (OR) to predict the risk factor for diabetes.

Conclusion: The hidden burden of diabetes in our locality is quite high, which, if not taken care, would result in a public health catastrophe.

Keywords: Diabetes, Dyslipidemia, Fasting plasma glucose, Glycosylated hemoglobin, Prediabetes.

How to cite this article: Patel S, Nanda R, Abraham J, Sahoo S, Ganguly A, Mohapatra E. Prediabetes and Undiagnosed Diabetes Mellitus: The Hidden Danger. Indian J Med Biochem 2017;21(2):91-95.

Source of support: Nil

Conflict of interest: None

 
9.  Original Article
Does Prolonged Storage of Serum Samples alter the Lab Results?
Devika Tayal, Mrinal Gupta, Binita Goswami
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:30-33] [No of Hits : 583]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0015 | FREE

ABSTRACT

Background: Sample storage for prolonged periods can lead to alterations in routine biochemical parameters.

Aim: This study was undertaken to observe the biochemical changes on serum samples when they are stored for prolonged periods at -20°C on different routine biochemical parameters.

Results: Significant changes were observed in serum glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and potassium levels after 72 hours of storage, which further deteriorate in 3 months. Rest of the parameters did not show any significant alteration.

Conclusion: Prolonged storage of samples lead to alterations in the analyte concentration in serum. Serum glucose, AST, ALT, creatinine, and potassium levels should be estimated within 72 hours if prolonged storage is unavoidable. In conclusion, all the parameters should be assayed within 24 hours to avoid misinterpretation of results and better patient care.

Keywords: Alanine transaminase, Aspartate transaminase, Biochemical parameters, Creatinine.

How to cite this article: Tayal D, Gupta M, Goswami B. Does Prolonged Storage of Serum Samples alter the Lab Results? Indian J Med Biochem 2017;21(1):30-33.

Source of support: Nil

Conflict of interest: None

 
10.  MEDICAL EDUCATION-ORIGINAL ARTICLE
Case-based Learning in Biochemistry: An Innovative Criterion-based Assessment Module for Effective Learning among MBBS Phase 1 Students in India
Vijayetha P Patil, Vidya S Patil, Shrirang P Kulkarni, Dhiraj J Trivedi, Vani C Axita, Rakesh T Muddaraddi, Praveen Kumar Shetty, Anil B Bargale, Pramod S Kamble, Deepti G Ingleshwar, AS Shilpasree
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:62-68] [No of Hits : 531]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0022 | FREE

ABSTRACT

Introduction: The main focus of case-based learning (CBL) is to integrate basic science material with clinical science. Case-based learning may be designed and introduced along with traditional didactic lectures in a way that it creates interest, promotes better understanding, active participation, and selfdirected learning. The student should be motivated to become a life-long learner.

Intervention: We have formulated an innovative criterion-based module for implementation and evaluation of MBBS phase 1 students. This includes framing of a time schedule for distribution of teaching hours based on multiple teaching tools and module for conducting CBL sessions. This will be assessed by evaluation of student performance using a newly developed valid assessment tool.

Materials and methods: For the CBL sessions, 100 students of phase 1 MBBS course were included. They were divided into 10 groups of 10 students each. Ten clinical cases were selected for CBL sessions. Each student was given two cases for discussion. Pretest was conducted before and posttest after the CBL session. The session was conducted as planned in the module. The performance of the students was analyzed. Feedback from the students and teachers were taken and analyzed for its utility and effectiveness.

Results: The performance of the students showed drastic improvement after the CBL sessions (p < 0.0001); 85% of the students and 80% of the teachers agreed that CBL helps in deep learning and critical thinking; 84% of students and 73% teachers felt that CBL makes subject interesting with good retention of contents and reasoning ability for their future medical practice.

Conclusion: Our newly formulated assessment tool stands appropriate, which can be included in the present curriculum. Both the teachers and the students were satisfied with this kind of teaching method. This innovative teaching module and our newly formulated assessment tool proved to be very effective for learning biochemistry.

Keywords: Assessment tool, Biochemistry, Case-based learning, Critical thinking, Curriculum planning, Multiple teaching tools, Structured cases, Student feedback, Teacher’s reflections, Teaching module.

How to cite this article: Patil VP, Patil VS, Kulkarni SP, Trivedi DJ, Axita VC, Mudaraddi RT, Shetty P, Bargale AB, Kamble PS, Ingleshwar DG, Shilpasree AS. Case-based Learning in Biochemistry: An Innovative Criterion-based Assessment Module for Effective Learning among MBBS Phase 1 Students in India. Indian J Med Biochem 2017;21(1):62-68.

Source of support: Nil

Conflict of interest: None

 
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