Indian Journal of Medical Biochemistry

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VOLUME 21 , ISSUE 1 ( January-June, 2017 ) > List of Articles


Establishment of Reference Intervals of Serum Hepatic Enzymes in Tertiary Care Hospital: A Pilot Study

P Shruthi Rai, Sukanya Shetty, Priya Patil, Roopa Bhandary

Citation Information : Rai PS, Shetty S, Patil P, Bhandary R. Establishment of Reference Intervals of Serum Hepatic Enzymes in Tertiary Care Hospital: A Pilot Study. Indian J Med Biochem 2017; 21 (1):22-24.

DOI: 10.5005/jp-journals-10054-0013

License: CC BY 3.0

Published Online: 01-12-2012

Copyright Statement:  Copyright © 2017; The Author(s).



Reference intervals are the most common decision-making tools for the interpretation of various biochemical reports. Age, sex, ethnicity, diet, physical, and socioeconomic conditions affect the physiology of population. Hence, it is necessary to set up the reference values that are applicable to a specific population rather than using the reference values established for other population-based studies or from the literature provided by the reagent kit manufacturers.

Aims and objectives

To establish the reference intervals of serum hepatic enzymes in tertiary care hospital.

Materials and methods

Hospital-based cross-sectional study was done involving 200 subjects. The subjects were randomly selected from the population, which included medical students, health professionals, and those attending different outpatient departments and health camps organized by the hospital for general checkups under health plan scheme. Serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT) were estimated by the International Federation of Clinical Chemistry-recommended kinetic method and GGT by enzymatic colorimetric method. To obtain reference interval, 97.5th percentile and 2.5th percentile were used.


The results for serum AST and GGT were found to be statistically significant. Hence separate reference intervals for both male and female were set up. Generalized reference intervals were established for serum ALT and ALP levels.


The present study revealed a significant difference in the reference intervals between males and females.

How to cite this article

Rai PS, Shetty S, Patil P, Bhandary R. Establishment of Reference Intervals of Serum Hepatic Enzymes in Tertiary Care Hospital: A Pilot Study. Indian J Med Biochem 2017;21(1):22-24.

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