With growing awareness of the link between diet and health and the problem of obesity, public concern over sugar levels in the diet is forcing a worldwide trend toward cutting down on sugar by using artificial sweeteners (AS).
To study the effect of increasing concentrations of sucralose (an AS) on glucose uptake in rat L6 myotubes.
Materials and methods
The L6 cell line from American type cell culture (ATCC) was grown in Dulbecco's Modified Eagle's Medium (DMEM) and differentiated into myotubes. The wells were exposed to either 0, 1 nM, 1 μM, or 1 mM of sucralose alone or with 10 nM insulin for 24 hours. Glucose uptake was studied after this period.
Significant decrease was seen between the insulin-stimulated basal glucose uptake and insulin-stimulated glucose uptake across all the concentrations of sucralose treatment.
Increased concentration of sucralose appears to decrease glucose uptake even on insulin stimulation.
It may not be beneficial to use sucralose in certain groups of people who have insulin resistance or are prone to it.
How to cite this article
Prakash SN, Shanthakumari J, Devanath A. Effect of Sucralose on Glucose Uptake in Rat L6 Myotubes. Indian J Med Biochem 2017;21(2):162-165.
Failure of sucrose replacement with the non-nutritive sweetener erythritol to alter GLP-1 or PYY release or test meal size in lean or obese people. Appetite 2016 Dec;107:596-603.
The effect of five artificial sweeteners on CaCO2, HT-29 and HEK-293 cells. Drug Chem Toxicol 2015 Oct;38(3):318-327.
Artificial sweeteners as a sugar substitute: are they really safe? Indian J Pharmacol 2016 May-Jun;48(3):237-240.
Soft drink consumption and risk of developing cardiometabolic risk factors and the metabolic syndrome in middle-aged adults in the community. Circulation 2007 Jul;116(5):480-488.
Fueling the obesity epidemic? Artificially sweetened beverage use and long-term weight gain. Obesity (Silver Spring) 2008 Aug;16(8):1894-1900.
Diet soda intake is associated with long-term increases in waist circumference in a biethnic cohort of older adults: the San Antonio Longitudinal Study of Aging. J Am Geriatr Soc 2015 Apr;63(4):708-715.
Artificial sweeteners and cancer risk in a network of case-control studies. Ann Oncol 2007 Jan;18(1):40-44.
The intake of intense sweeteners — an update review. Food Addit Contam 2006 Apr;23(4):327-338.
Sugar substitutes: health controversy over perceived benefits. J Pharmacol Pharmacother 2011 Oct;2(4):236-243.
Biological fate of low-calorie sweeteners. Nutr Rev 2016 Nov;74(11):670-689.
Sucralose, a synthetic organochlorine sweetener: overview of biological issues. J Toxicol Environ Health B Crit Rev 2013 Sep;16(7):399-451.
Glycemic effect of a single high oral dose of the novel sweetener sucralose in patients with diabetes. Diabetes Care 1996 Sep;19(9):1004-1005.
Lack of effect of sucralose on glucose homeostasis in subjects with type 2 diabetes. J Am Diet Assoc 2003 Dec;103(12):1607-1612.
Repeated dose study of sucralose tolerance in human subjects. Food Chem Toxicol 2000 Feb;38(Suppl 2):S123-S129.
Effect of the artificial sweetener, sucralose, on small intestinal glucose absorption in healthy human subjects. Br J Nutr 2010 Sep;104(6):803-806.
Effects of oral ingestion of sucralose on gut hormone response and appetite in healthy normal-weight subjects. Eur J Clin Nutr 2011 Apr;65(4):508-513.
Effects of different sweet preloads on incretin hormone secretion, gastric emptying, and postprandial glycemia in healthy humans. Am J Clin Nutr 2012 Jan;95(1):78-83.
Short-term consumption of sucralose, a nonnutritive sweetener, is similar to water with regard to select markers of hunger signaling and short-term glucose homeostasis in women. Nutr Res 2011 Dec;31(12):882-888.
Artificial sweeteners have no effect on gastric emptying, glucagon-like peptide-1, or glycemia after oral glucose in healthy humans. Diabetes Care 2013 Dec;36(12):e202-e203.
Artificial sweetener use and one-year weight change among women. Prev Med 1986 Mar;15(2):195-202.
Patterns of weight change and their relation to diet in a cohort of healthy women. Am J Clin Nutr 1990 Jun;51(6):1100-1105.
Adults with healthier dietary patterns have healthier beverage patterns. J Nutr 2006 Nov;136(11):2901-2907.
Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature 2014 Oct;514(7521):181-186.
Sucralose affects glycemic and hormonal responses to an oral glucose load. Diabetes Care 2013 Sep;36(9):2530-2535.
Effects of the non-nutritive sweeteners on glucose metabolism and appetite regulating hormones: systematic review of observational prospective studies and clinical trials. PLoS One 2016 Aug;11(8):e0161264.