Citation Information :
Jabir FA, Hamzah SK. SOX17 and RASSAF1A Promoters Methylation in Circulation Tumor Cell and Cell Free Deoxyribonucleic Acid Isolated from Plasma in Breast Cancer. Indian J Med Biochem 2018; 22 (2):108-113.
Purpose: The study was to evaluate SOX17, and RASSEF 1A promoters methylation levels in patients with malignant breast tumors compared with healthy women.
Materials and methods: Fifty women with breast cancer (range 26 to 75 years) were included in this study. They were admitted at Al-Diwaniyah Teaching Hospital, Iraq, compared with 25 women as the control. We obtained plasma samples from 50 patients (breast cancer) stages II, III, IV and normal (healthy women). Cell-free deoxyribonucleic acid (DNA) (cfDNA) extracted and methylation analysis for SOX17 and RASSEF1A gene.
Results: We found different methylation pattern of the RASSEF1A gene in plasma samples between patients with breast cancer and control (p < 0.05) ranging in all cases 30 (60%) and unmethylation in 20 (40%), hyper methylation frequency in all studied cases [13 (44.8%) for II stage, 12 (80%) III stage and 4 (66.6%) IV stage of breast cancer in this study association between the hypermethylation was significant with age. 50 11.4 (71.2%) and < 50 26 (76%)] than normal cases, we found different methylation pattern of the SOX17 gene in plasma samples between patients with breast cancer and control (p < 0.05) ranging in all cases 29 (58%) and unmethylation in 21 (42%),hyper methylation frequency in all studied cases 11 (37.9%) for II stage, 13 (86.6%) III stage and 5 (83.3%) IV stage of breast cancer in this study association between the hyper methylation was significant with age (. 50 13 (81.2%) and < 50 23 (67%) than normal cases also this study association between the hypermethylation was significant with menopause (premenopause 6 (66%), post menopause 31 (75.6%) than the control group (0%).
Conclusions: Our data indicate that methylation of the RASSAF1A and SOX17 genes are the frequent event in primary breast cancer and that it plays a major role in the silencing of the expression of this gene during tumor development.
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