Citation Information :
V KV, Ramasamy R, Hanifah M, M L. Circulating MiR-1 Levels in Acute Myocardial Infarction and Its Significance in Differential Diagnosis and Prognosis. Indian J Med Biochem 2019; 23 (1):174-177.
Background: Acute myocardial infarction (AMI) is a condition where there is a formation of thrombus which reduces the blood flow in the coronary arteries resulting in coronary vasospasm, further leading to cardiac muscles dysfunction or even death. Quick and accurate diagnosis of AMI plays a major role in the management and prognosis of this disease. Circulating miRNA has been a promising cardiac biomarker for AMI as well as cardiovascular disease in recent years. In the circulation miR 1 overexpressed when there is any damage in the cardiac cell. A very few articles documented the use of miR-1 as a diagnostic marker in AMI. The aim of the study was to find the diagnostic and prognostic role of miR-1 in AMI.
Methodology: In this study, 62 AMI patients in the age group of 30–75 years and 30 healthy controls were included. Blood samples were collected at a different time of admission, 6 hours, 12 hours, 24 hours and 72 hours for the subject. Serum was separated immediately for the analysis of CK (NAC) and CK MB, Hs troponin T and micro-RNA-1 was quantification by RT-PCR. To study the kinetics of miR-1 repeated measure of analysis of variance (ANOVA) was analyzed. To find the diagnostic utility AUC, likelihood and odd\'s ration was performed.
Results: The miR-1 expression is expressed in a change in fold 27.36, 57.17, 43.55, 32.50 and 24.09 fold increase at the different time. The study revealed that the sensitivity 69%, specificity 66%, LR was 1.59 and OR 4.04 in the differential diagnosis of AMI.
Conclusion: In a total expression of miR-1 showed statistical significance in different time internal which will help us to use in the differential diagnosis of AMI.
Ferreira GMT de M, Correia LC, Reis H, et al. Maior letalidade e morbidade por infarto agudo do miocárdio em hospital público, em Feira de Santana-Bahia. Arq Bras Cardiol 2009 Aug;93(2): 97-104.
Roger VL. Epidemiology of Myocardial Infarction. Med Clin North Am. 2007 Jul;91(4):537-552.
Carvalho G, Rassi S. The Prognostic Value of CK-MB in Acute Myocardial Infarction in Developing Countries: A Descriptive Study. Angiol Open Access [Internet]. 2016 [cited 2017 Nov 3];4(3). Available from: http:// www.esciencecentral.org/journals/the-prognostic-value-of-ckmb-inacute- myocardial-infarction-in-developingcountries-a-descriptivestudy- 2329-9495-1000183.php?aid=79371
Slavich M, Patel RS. Coronary artery spasm: Current knowledge and residual uncertainties. Int J Cardiol Heart Vasc 2016 Jan 23;10: 47-53.
Chandrasekaran B, Kurbaan AS. Myocardial infarction with angiographically normal coronary arteries. J R Soc Med 2002 Aug;95(8):398-400.
Kumar A, Cannon CP. Acute Coronary Syndromes: Diagnosis and Management, Part I. Mayo Clin Proc 2009 Oct;84(10):917-938.
Al-Hadi HA, Fox KA. Cardiac Markers in the Early Diagnosis and Management of Patients with Acute Coronary Syndrome. Sultan Qaboos Univ Med J 2009 Dec;9(3):231-246.
Daubert MA, Jeremias A. The utility of troponin measurement to detect myocardial infarction: review of the current findings. Vasc Heal Risk Manag 2010;6:691-699.
Cheng Y, Tan N, Yang J, et al. A translational study of circulating cellfree microRNA-1 in acute myocardial infarction. Clin Sci Lond Engl
2010 Apr 20;119(2):87-95.
Felekkis K, Touvana E, Stefanou C, et al. microRNAs: a newly described class of encoded molecules that play a role in health and disease. Hippokratia 2010;14(4):236-240.
Cannell IG, Kong YW, Bushell M. How do microRNAs regulate gene expression? Biochem Soc Trans. 2008 Dec;36(Pt 6):1224-1231.
Glinge C, Clauss S, Boddum K, et al. Stability of Circulating Blood- Based MicroRNAs – Pre-Analytic Methodological Considerations. PLoS One [Internet]. 2017 Feb 2;12(2). Available from: https://www. ncbi.nlm.nih.gov/pmc/articles/PMC5289450/
Viereck J, Thum T. Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury. Circ Res. 2017 Jan 20;120(2):381-399.
Ai J, Zhang R, Li Y, Pu J, Lu Y, Jiao J, et al. Circulating micro RNA-1 as a potential novel biomarker for acute myocardial infarction. Biochem Biophys Res Commun. 2010 Jan 1;391(1):73-77.
Chen X, Ba Y, Ma L, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008 Oct;18(10):997-1006.
D'Alessandra Y, Devanna P, Limana F, et al. Circulating microRNAs are new and sensitive biomarkers of myocardial infarction. Eur Heart J. 2010 Nov;31(22):2765-2773.
Xinran Xu FD. The Role of miR-1 in the Heart: From Cardiac Morphogenesis to Physiological Function. Hum Genet Embryol [Internet]. 2014 [cited 2017 Nov 29];04(01). Available from: https://www.omicsonline.org/openaccess/ the-role-of-mir-in-the-heart-from-cardiac-morphogenesis-tophysiological- function-2161-0436.1000119.php?aid=26295