Indian Journal of Medical Biochemistry

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VOLUME 23 , ISSUE 1 ( January-April, 2019 ) > List of Articles

Original Article

Stability of Common Biochemical Analytes in Serum when Subjected to Changes in Storage Conditions and Temperature

P Kughapriya, JA Elanchezhian

Keywords : Accuracy, Aliquot, Analytes, Preanalytical, Precision

Citation Information : Kughapriya P, Elanchezhian J. Stability of Common Biochemical Analytes in Serum when Subjected to Changes in Storage Conditions and Temperature. Indian J Med Biochem 2019; 23 (1):178-181.

DOI: 10.5005/jp-journals-10054-0080

License: CC BY-NC 4.0

Published Online: 01-04-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Aim: The aim of the study was to assess the effect of storage time and temperature on the test results of 8 common biochemical analytes in serum. Materials and methods: Five mL of blood was collected from apparently healthy 80 volunteers in a clot activator tube without anticoagulant. Serum was separated after allowing the sample to clot for 30 minutes at room temperature. Separated serum was made into four aliquots. The first part analyzed within two hours act as a baseline value, while the other three aliquots were stored at room temperature for four hours, at 2–8° C for four hours and at 2–8° C for 24 hours, respectively. Results: Baseline serum values of common biochemical analytes analyzed within two hours of sample collection on comparison with the same sample stored at room temperature for four hours showed a significant change in the values of glucose, urea, creatinine, total bilirubin, albumin, and total protein. Also the values of glucose, urea and albumin were significantly changed on comparing the baseline sample with the sample that is stored for four hours at 2–8° C. Finally we compared the baseline values with the sample stored at 2–8° C for 24 hours and found a significant change among the values of glucose, total bilirubin, and albumin. Other analytes were found to be stable. Conclusion: According to our study, storage of common biochemical analytes in serum like glucose, urea, bilirubin, and albumin is not advisable. Serum creatinine and total protein were stable on refrigeration. Total cholesterol and triglycerides were found to be stable. Clinical significance: In the context of patient centered approach to the delivery of healthcare services, clinical laboratories play a vital role. Despite the improvements made in the laboratory, errors still occur due to inadequate insight about the importance of sample collection and storage procedures. Errors in sample handling affects test report which diminishes the confidence in healthcare services and damages an institution's reputation. Significant interdepartmental cooperation is very essential for identification and proper management of preanalytical errors which inturn will help to make valuable medical decision and effective patient care.

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