Indian journal of Medical Biochemistry

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VOLUME 23 , ISSUE 1 ( January-April, 2019 ) > List of Articles

Original Article

Comparative Study of Transudate and Exudate Pleural Fluid Using C-reactive Protein, Uric Acid and Malondialdehyde as Markers

Malathi M

Keywords : C-reactive protein, Malondialdehyde, Uric acid

Citation Information : M M. Comparative Study of Transudate and Exudate Pleural Fluid Using C-reactive Protein, Uric Acid and Malondialdehyde as Markers. Indian J Med Biochem 2019; 23 (1):193-196.

DOI: 10.5005/jp-journals-10054-0084

License: CC BY-NC 4.0

Published Online: 01-04-2019

Copyright Statement:  Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction: Pleural effusion is one of the most important debilitating disorders caused by various pathological conditions. It is a common clinical disorder and is either a manifestation or a complication of one or other respiratory of non-respiratory diseases. Diagnosing the type of effusion as an exudate or transudate is equally important in identifying the cause and treating accordingly to the type of pleural effusion. Hence, the use of light\'s criteria to separate transudate from exudate has been generally the admitted first step in any study of pleural effusion of the unknown cause. As the pleural effusion may increase the local oxidative stress so that pleural fluid can be analyzed for the oxidative stress marker as one of the tool additives with the light\'s criteria for the better differentiating the exudative from transudative type of effusion. Aim: Estimate levels of malondialdehyde (MDA), C-reactive protein (CRP) and uric acid in pleural fluid and compare the levels in exudative and transudative pleural fluid. Materials and methods: The samples for the study were collected after taking informed consent. The pleural tapping was done under all required aseptic precaution. It was divided into two groups—transudative pleural effusion (group 1) and exudative pleural effusion (group 2) by using Light\'s criteria. Samples were analyzed for the MDA, CRP and uric acid. Results and conclusion: MDA and CRP values have shown significantly increased levels in the pleural fluid of exudative type compared to transudative, but pleural uric acid levels were lower in exudative. However, there was no much difference between these parameters in serum levels among groups. The present study showed that oxidative stress is more in the exudates compared to transudates, probably due to the higher production of reactive oxygen species; it may serve as markers for differentiating between exudates and transudates.


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  1. Light RW. Pleural Effusions: The Diagnostic Separation of Transudates and Exudates. Ann Intern Med. American College of Physicians 1972;77(4):507-516.
  2. Storey D, Dines D, Coles D. Pleural effusion: A diagnostic dilemma. JAMA 1976;236(19):2183-2186.
  3. Alfredo C, Luis H CT. Evaluation of different criteria for the separation of pleural transudates from exudates. Chest 1993;104(2):399-415.
  4. Jain A, Kumar S, Jain R, B et al. A study of uric acid - a new biochemical marker for the differentiation between exudates and transudates in a pleural effusion cases a study of uric acid - a new biochemical marker for the differentiation between. Natl J community Med 2015;5(2):204- 208.
  5. Usha Kiran P, Komala P. Comparison of transudates and exudates using malondialdehyde and lipid profile. Int J Biol Med Res 2015;6(1): 4737-4740.
  6. Gupta KB. Evaluation of pleural fluid and serum MDA levels in differentiating transudative from exudative pleural effusions. Ind J Tub. 2002;49:97-100.
  7. Papageorgiou E, Kostikas K, Kiropoulos T. Increased Oxidative Stress in Exudative Pleural Effusions. Chest 2005;128(5):3291-3297.
  8. Mangaraj M, Kumari S, Nanda R, et al. Pleural fluid MDA and serumeffusion albumin gradient in pleural effusion. Indian J Clin Biochem 2008;23(1):81-84.
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