The advantage of Accreditation is quality test reports and customer satisfaction. Technicians are integral part of medical testing laboratories. So they should have commitment towards acquiring and updating sound knowledge about good laboratory practices. Aim: To assess the impact of accreditation on awareness and knowledge of technician’s about laboratory quality measures in tertiary care hospital. Material and Method: We prepared a questionnaire based on quality management system (QMS), Pre-Analytical, Analytical, Post-analytical examination and laboratory safety. Total thirty technicians appeared for pretest before accreditation, followed by various training sessions and NABL accreditation. A post test was conducted after accreditation for the same. Result: The data of pretest and post-test performance was analyzed by SPSS software. The result shows there was significant (p < 0.001) increase in the score of the marks among technicians in post-test (20.67±3.67) compared to pre-test (10.21 ±4.39). Conclusion: In present study we observed suboptimal knowledge & awareness in technicians regarding QMS. This was found enhanced after Accreditation process & continuous training sessions. This will be helpful to improve their skill to sustain quality laboratory services.