Evaluating Blood Glucose-6-Phosphate Dehydrogenase Activity with Oxidative Stress: A Study in Uncomplicated Type 2 Diabetes Mellitus Patients
Ruchika Priyadarsini, Pramila K Mishra, Nirupama Devi, Rasmita K Padhy
Citation Information :
Priyadarsini R, Mishra PK, Devi N, Padhy RK. Evaluating Blood Glucose-6-Phosphate Dehydrogenase Activity with Oxidative Stress: A Study in Uncomplicated Type 2 Diabetes Mellitus Patients. Indian J Med Biochem 2020; 24 (1):4-8.
Background: Diabetes mellitus (DM) is chronic hyperglycemia condition affecting multiple organs due to metabolic disorder. Insulin secretion, function, or both are affected for which one of the factors attributed is due to increased free radical activity. Nicotinamide adenine dinucleotide phosphate (NADPH) produced in HMP shunt pathway is regulated by the rate-limiting glucose-6-phosphate dehydrogenase (G6PD). When there is an imbalance between the production of reactive oxygen species and the antioxidant system that detoxifies, then it is called oxidative stress. This pathway is regulated by the reductant concentration of NADPH. Aims and objectives: The current study was taken up to evaluate and correlate oxidative stress and insulin resistance with G6PD activity in type 2 DM (T2DM) patients. Materials and methods: A total of 100 (76 males 24 females) T2DM patients with equal age- and sex-matched healthy controls were selected for the study. Glucose-6-phosphate dehydrogenase was measured by chemical method in semiauto analyzer. Total oxidative stress measured as ferrous oxidation in xylenol orange and total antioxidant capacity estimated as ferric-reducing ability of serum by spectrophotometer. Glucose was measured by glucose oxidase-peroxidase method in an autoanalyzer. SPSS Version 20 software was used for statistical analysis. Results and observations: Increased serum G6PD levels were found in DM patients which significantly correlates with the increase of oxidative stress and high glucose levels (p value < 0.01). Conclusion: Estimation of blood G6PD activity may be used as a test to know the extent of oxidative status in DM patients for its implications in further clinical complications.
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