Testosterone levels are very important to be maintained in males, but the level reduces as age advances. If symptoms of severe or chronic low testosterone are present, hormone deficiency is amenable, it may be caused by an active disease or condition. In this review, we have tried to summarize how testosterone biosynthesis, regulation, as well as metabolism occurs in the human body. How it plays numerous roles: starting from intrauterine life to an advanced age, various factors such as age, weight, sleep, food habits, etc., affecting testosterone levels in the body are also discussed. These factors are very important in understanding the clinical implications of testosterone on the health of an individual. We also summarize various assays that are currently done for testosterone evaluation.
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