Citation Information :
Patil SM, Bankar MP. Study of Correlation between the Paraoxonase 1 (PON1) Activity and Lipid Profile in Various Types of Coronary Heart Disease. Indian J Med Biochem 2021; 25 (2):65-70.
Aim and background: Coronary heart disease (CHD) is the major cause of mortality and morbidity worldwide. Human serum paraoxonase-1 is a high-density lipoprotein (HDL)-bound enzyme exhibiting anti-atherogenic properties.
Objective: The current study aimed to determine the serum paraoxonase-1 activity and lipid profile with CHD in addition to correlate the relationship between serum high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein (LDL) cholesterol, and paraoxonase-1 level in patients with CHD.
Materials and methods: In the present case-control study, 265 with coronary artery disease (age range 26–75) and 120 age- and sex-matched healthy controls were recruited. Serum paraoxonase activity was measured spectrophotometrically by using phenylacetate as substrate by kinetic assay while lipid profile was analyzed by an enzymatic method by cholesterol oxidase peroxidase (CHOD-PAP) method of total cholesterol and HDL-C and glycerol 3-phosphate oxidase (GPO-PAP) method of triglyceride. Values were expressed as mean ± standard deviation and data from patients and controls were compared by using the Student's “t” test.
Results: Serum paraoxonase-1 activity was significantly (p < 0.01) decreased in CHD when compared with healthy controls. Similarly, there was a significant difference between mean values of paraoxonase-1 when all CHD groups compared with each other (p < 0.01). Correlation between paraoxonase-1 vs HDL-C was positive in stable angina (Karl Pearson's correlation coefficient r = 0.04671), myocardial infarction (MI) (r = 0.2643), and controls (r = 0.06384) and it was negative in unstable angina (UA) (r = −0.098). There was negative correlation between paraoxonase-1 vs low-density lipoprotein (LDL)-cholesterol in stable angina (r = −0.0291), MI (r = −0.2265), and control group (r = −0.1767), and was positive in UA (r = 6185).
Conclusion: Low paraoxonase-1 may be reducing the capacity of HDL to prevent the oxidation of LDL cholesterol, therefore, lead to CHD. So interventional means of dietary antioxidants to conserve or even to raise paraoxonase-1 activity may contribute to attenuation of atherogenesis.
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