Correlation of Protein Carbonyl and Protein Thiols in Oxidative Stress-induced Senescence of Red Blood Cell Membrane in Type 2 Diabetes Mellitus
Veerabhadra Goud GK, Dinesh Javarappa
Keywords :
Diabetes mellitus (DM), Oxidative stress, Protein carbonyl, Protein thiols, Reactive oxygen species.
Citation Information :
GK VG, Javarappa D. Correlation of Protein Carbonyl and Protein Thiols in Oxidative Stress-induced Senescence of Red Blood Cell Membrane in Type 2 Diabetes Mellitus. Indian J Med Biochem 2018; 22 (2):143-146.
Introduction: Diabetes mellitus (DM) is an endocrine dysfunction, which is associated with metabolic disease. Wherein chronic hyperglycemia presets the oxidative process which in turn biochemically leads to advanced glycation. The aim is to study the changes caused by oxidative stress upon the red blood cell (RBC) membrane composition by protein carbonyl and protein thiols in type 2 DM (T2DM) in comparison with that of controls. Materials and methods: The study includes two groups, consists of 100 cases of T2DM and 100 healthy subjects as controls. Blood samples were collected under strict aseptic precautions as per the criteria and estimation of fasting blood sugar (FBS), hemoglobin A1c (HbA1c), protein carbonyl with RBC membrane ghost preparation and estimation of protein thiols were done. Results: It was found that there was significant increase of protein carbonyl in cases (1.20 ± 0.08), in comparison to normal controls (0.90 ± 0.06) with a statistical significance of (p < 0.001) along with Protein thiols of RBC membrane which was significantly decreased (1.42 ± 0.10) in cases in comparison to normal controls. (2.12 ± 0.12) with a statistical significance of (p < 0.001). In our study, the positive correlation of protein carbonyl and protein thiols was established with 74% of cases of type 2 DM falling into a HbA1C control group of 7 to 8% indicating the increased protein carbonyl and decreased protein thiols are early indicators of progressive diabetic changes. Conclusion: Changes in RBC membrane of persons with T2DM acts as an early indicator by means of an increase in protein carbonyl and decrease in protein thiols, and this will further lead to progressive diabetic changes.
Tiwari BK, Pandey KB, Abidi AB, Rizvi SI. Markers of Oxidative Stress during Diabetes Mellitus. Journal of Biomarkers [Internet]. Hindawi Limited; 2013;2013:1–8.
Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and Diabetes: A Review. Int. J. Pharma Applications. 2010;1: 38-45.
Mousa SA, Oxidative stress in DMs. Roman J Biophys. 2008;18(3);222-236.
Kalaivanam KN, Kumar SN, Bheemasen R, Mahendran BK. Role of protein oxidation and lipid peroxi dation markers status in NIDDM patients. World Journal of Pharmacy and Pharmaceutical Sciences. 2014;3(6):1510-1520.
Fujino T, Ando K, Beppu M, Kikkugawa K. Enzymatic removal of protein aggregates from erythrocyte membranes. J.Biochem. 2000;127(6):1081-1086.
Celedone G, Rodriguez I, Espana J, Lissi E. Contribution of Hemoglobin and membrane constituents modification to human erythrocyte damage produced by peroxyradicals of different charge and hydrophobicity. Free Radic. Res 2001;34:17-31.
Burtis CA. Carbohydrates in Sacks DD ednt. Clinical chemistry. Chapter 22. 6th edition. Saunders 2008;390-391.
Gowenlock AH, McMurray JR, McLauchen DM, Tests in disorders of Glucose metabolism. In: Weiner K edn. Practical clinical Biochemistry. Chapter 25. 6th edition. CBS Publishers. 1996:333-349.
Dodge JT, Mitchell C. Hanahan DJ, The preparation and chemical characteristics of Hemoglobin free ghosts of human erythrocyte. Arch Biochem Biophys. 1963:100119-100213.
Levine RL, William JA, Stadtman ER, Shacter E. Carbonyl assays for determination of oxidativley modified proteins, Methods Enzymol. 1994;233:346-357.
Habeeb AF. Reaction of protein sulfhydryl groups with Ellman's reagent. Reaction of protein sulfhydryl groups with Ellman's reagent. Methods Enzymol. 1972;25:457-464.
Stryer L. Portrait of an allosteric protein. Biochemistry. 1995:154.
Mallick AK, Maradi R, Joshi VR, Shorey G, Ahsan M. Study on malondialdehyde as a marker of lipid peroxidation in male and female patients with type 2 diabetes mellitus. International Journal of Pharmaceutical Sciences Review and Research. 2011;8(2):198-201.
Almogbel E, Rasheed N. Protein Mediated Oxidative Stress in Patients with Diabetes and its Associated Neuropathy: Correlation with Protein Carbonylation and Disease Activity Markers. Journal of clinical and diagnostic research: JCDR. 2017 Feb;11(2):BC21-BC25.
Deokar P, Jagtap A, Yerawar C. Correlation of protein carbonyl and MDA in diabetes and its complications. Indian Journal of Basic and Applied Medical Research. 2016;5(2):284-289..
Sagare AA, Trivedi DJ, Patil VS, Kulkarni SP. Protein Carbonyl & Microalbuminuria in Type 2 Diabetes Mellitus. Indian Journal of Basic and Applied Medical Research; 2012 Dec;2(5):399-404.
Gabreanu GR, Angelescu S. Erythrocyte membrane in type 2 diabetes mellitus. Discoveries [Internet]. Applied Systems, srl; 2016 Jun 30;4(2):e60.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurment with the Folin phenol reagent. J Biol Chem. 1951. Nov;193(1):265-275.