Citation Information :
Kughapriya P, Elanchezhian J. Stability of Common Biochemical Analytes in Serum when Subjected to Changes in Storage Conditions and Temperature. Indian J Med Biochem 2019; 23 (1):178-181.
Aim: The aim of the study was to assess the effect of storage time and temperature on the test results of 8 common biochemical analytes in serum. Materials and methods: Five mL of blood was collected from apparently healthy 80 volunteers in a clot activator tube without anticoagulant. Serum was separated after allowing the sample to clot for 30 minutes at room temperature. Separated serum was made into four aliquots. The first part analyzed within two hours act as a baseline value, while the other three aliquots were stored at room temperature for four hours, at 2–8° C for four hours and at 2–8° C for 24 hours, respectively. Results: Baseline serum values of common biochemical analytes analyzed within two hours of sample collection on comparison with the same sample stored at room temperature for four hours showed a significant change in the values of glucose, urea, creatinine, total bilirubin, albumin, and total protein. Also the values of glucose, urea and albumin were significantly changed on comparing the baseline sample with the sample that is stored for four hours at 2–8° C. Finally we compared the baseline values with the sample stored at 2–8° C for 24 hours and found a significant change among the values of glucose, total bilirubin, and albumin. Other analytes were found to be stable. Conclusion: According to our study, storage of common biochemical analytes in serum like glucose, urea, bilirubin, and albumin is not advisable. Serum creatinine and total protein were stable on refrigeration. Total cholesterol and triglycerides were found to be stable. Clinical significance: In the context of patient centered approach to the delivery of healthcare services, clinical laboratories play a vital role. Despite the improvements made in the laboratory, errors still occur due to inadequate insight about the importance of sample collection and storage procedures. Errors in sample handling affects test report which diminishes the confidence in healthcare services and damages an institution's reputation. Significant interdepartmental cooperation is very essential for identification and proper management of preanalytical errors which inturn will help to make valuable medical decision and effective patient care.
Kaplan LA. Determination and Application of Desirable Analytical Performance Goals: the ISO/TC 212 approach. Scandivian J. Clin. Lab Investi. 1999;59:479-482.
Plebani M, Carraro P. Mistakes in a stat laboratory: types and frequency. Clin Chem 1997;43:1348-1351.
Carraro P, Plebani M. Errors in a stat laboratory: types and frequencies 10 years later. Clin Chem 2007;53:1338-1342.
Kouri T, Siloaho M, Pohjavaara S, et al., Pre-analytical factors and measurement uncertainty, Scand J Clin Lab Invest 2005;65:463-476.
Arzoumanian L. BD Technical Services News Bulletin 2003;2(2).
Burtis CA, Ashood ER, Burns DE. Tietz Textbook of clinical chemistry and molecular diagnostics. Fifth edition. Elsevier 2012;145-161.
Timms JF, Arslan-Low E, Gentry-Maharaj A, et al. Clin Chem. PubMed. 2007;53(4):645-656.
CLSI document H 18-A3. Procedures for the Handling and Processing of Blood Specimens; Approved Guideline. 3rd edn. 2004.
Selvakumar C, Madhubala V. Effect of sample storage and time delay (delayed processing) on analysis of common clinical biochemical parameters. International Journal of Clinical Biochemistry and Research, July-September 2017;4(3):295-298.
Cuhadar S, Atay A, Koseoglu M, et al. Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions. Biochemia Medica 2012; 22(2):202- 214.
Bruns DE, William C. Knowler. Stabilization of Glucose in Blood Samples: Why It Matters. Clin Chem 2009 Mar;55(5):850-852.
Stahl M, Jorgensen LGM, Hyltoft Petersen P, et al. Optimization of preanalytical conditions and analysis of plasma glucose. Impact of the new WHO and ADA recommendations on diagnosis of diabetes mellitus. Scand J Clin Lab Invest 2001;61:169-180.
Chan AYW, Swaminathan R, Cockram CS. Effectiveness of sodium fluoride as a preservative of glucose in blood. Clin Chem 1989;35:315- 317.
Pahwa M, Menaka K, Minakshi, et al. Effect of storage time and temperature on serum clinical biochemistry analytes. BCAIJ, 2015;9(4):150-156.
Shepherd J, Warner MH, Kilpatrick ES. Stability of creatinine with delayed separation of whole blood and implications for eGFR. Ann Clin Biochem 2007;44:384-387.
Kachhawa K, Kachhawa P, Varma M, et al. Study of the Stability of Various Biochemical Analytes in Samples Stored at Different Predefined Storage Conditions at an Accredited Laboratory of India. J Lab Physicians 2017 Jan-Mar; 9(1):11-15.
Comstock GW, Burke AE, Norkus EP, et al. Effects of repeated freezethaw cycles on concentrations of cholesterol, micronutrients, and hormones in human plasma and serum. ClinChem 2001; 47:139-142.
Sofronescu AG, Loebs T, Zhu Y. Effects of temperature and light on the stability of bilirubin in plasma samples. Clin Chim Acta. 2012 Feb;18;413(3-4):463-466.
Lawson NS, Haven GT, Moore TD. Long-term stability of enzymes, total protein, and inorganic analytes in lyophilized quality control serum. Am J Clin Pathol. 1977 Jul; 68(1 Suppl):117-129.
Mitchell BL, Yasui Y, Li CI, et al. Cancer Inf. PubMed. 2005;1(1):98-104.
Rai AJ, Gelfand CA, Haywood B, et al. Proteomics. PubMed. 2005;5(13):3262-3277.