Indian Journal of Medical Biochemistry

Register      Login

VOLUME 24 , ISSUE 1 ( January-April, 2020 ) > List of Articles


Evaluating Blood Glucose-6-Phosphate Dehydrogenase Activity with Oxidative Stress: A Study in Uncomplicated Type 2 Diabetes Mellitus Patients

Ruchika Priyadarsini, Pramila K Mishra, Nirupama Devi, Rasmita K Padhy

Citation Information : Priyadarsini R, Mishra PK, Devi N, Padhy RK. Evaluating Blood Glucose-6-Phosphate Dehydrogenase Activity with Oxidative Stress: A Study in Uncomplicated Type 2 Diabetes Mellitus Patients. Indian J Med Biochem 2020; 24 (1):4-8.

DOI: 10.5005/jp-journals-10054-0134

License: CC BY-NC 4.0

Published Online: 03-11-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Background: Diabetes mellitus (DM) is chronic hyperglycemia condition affecting multiple organs due to metabolic disorder. Insulin secretion, function, or both are affected for which one of the factors attributed is due to increased free radical activity. Nicotinamide adenine dinucleotide phosphate (NADPH) produced in HMP shunt pathway is regulated by the rate-limiting glucose-6-phosphate dehydrogenase (G6PD). When there is an imbalance between the production of reactive oxygen species and the antioxidant system that detoxifies, then it is called oxidative stress. This pathway is regulated by the reductant concentration of NADPH. Aims and objectives: The current study was taken up to evaluate and correlate oxidative stress and insulin resistance with G6PD activity in type 2 DM (T2DM) patients. Materials and methods: A total of 100 (76 males 24 females) T2DM patients with equal age- and sex-matched healthy controls were selected for the study. Glucose-6-phosphate dehydrogenase was measured by chemical method in semiauto analyzer. Total oxidative stress measured as ferrous oxidation in xylenol orange and total antioxidant capacity estimated as ferric-reducing ability of serum by spectrophotometer. Glucose was measured by glucose oxidase-peroxidase method in an autoanalyzer. SPSS Version 20 software was used for statistical analysis. Results and observations: Increased serum G6PD levels were found in DM patients which significantly correlates with the increase of oxidative stress and high glucose levels (p value < 0.01). Conclusion: Estimation of blood G6PD activity may be used as a test to know the extent of oxidative status in DM patients for its implications in further clinical complications.

PDF Share
  1. Wild S, Roglic G, Green A, et al. Global prevalence of diabetes estimate for the year 2000 and projection for 2030. Diabetes Care 2004;27(5):1047–1053. DOI: 10.2337/diacare.27.5.1047.
  2. Agrawal R, Rath B, Saha K, et al. Drug utilization pattern of antidiabetic agents in a tertiary care hospital of western Odisha, India. Int J Basic Clin Pharmacol 2016;5(5):2222–2226. DOI: 10.18203/2319-2003.ijbcp20163265.
  3. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414(6865):813–820. DOI: 10.1038/414813a.
  4. Buse MG. Hexosamines, insulin resistance, and the complications of diabetes: current status. Am J Physiol Endocrinol Metab 2006;290(1):1–8. DOI: 10.1152/ajpendo.00329.2005.
  5. Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. Diabetes 2005;54(6):1615–1625. DOI: 10.2337/diabetes.54.6.1615.
  6. Mousa SA. Oxidative stress in diabetes mellitus. Romanian J Biophys 2008;18(3):225–236.
  7. Fatokun AA, Stone TW, Smith RA. Oxidative stress in neurodegeneration and available means of protection. Front Biosci 2008;13(13): 3288–3311. DOI: 10.2741/2926.
  8. Forbes JM, Coughlan MT, Cooper ME. Oxidative stress as a major culprit in kidney disease in diabetes. Diabetes 2008;57(6):1446–1454. DOI: 10.2337/db08-0057.
  9. Lenzen S. Oxidative stress the vulnerable beta cell. Biochem Soc Trans 2008;36(Pt 3):343–347. DOI: 10.1042/BST0360343.
  10. Lubos E, Handy DE, Loscalzo J. Role of oxidative stress & nitric oxide in atherosclerosis. Front Biosci 2008;13(13):5323–5344. DOI: 10.2741/3084.
  11. Thomas SR, Witting PK, Drummond GR. Redox control of endothelial function & dysfunction: molecular mechanism & therapeutic opportunities. Antioxidant Redox Signals 2008;10(10):1713–1765. DOI: 10.1089/ars.2008.2027.
  12. Frederick WM, Bosch KS, Jong DJ, et al. Post translational regulation of glucose-6-phosphate dehydrogenase activity in (pre) neoplasti lesions in rat liver. J Histochem Cytochem 2003;51(1):105–112. DOI: 10.1177/002215540305100112.
  13. Stanton RC. Glucose-6- phosphate dehydrogenase, NADPH & cell survival. IUBMB Life 2012;64(5):362–369. DOI: 10.1002/iub. 1017.
  14. Brauwald E, Fauci AS, Longo DL, et al. Diabetes Mellitus. Harrison's Principle of Internal Medicine. 17th ed., (2), Newyork: MC Graw Hill; 2008. pp. 2275–2304.
  15. Alberti KGMM, Zimmet P, Shaw J. International diabetes federation: a consensus on type 2 diabetes prevention. Diabet Med 2007;24(5): 451–463. DOI: 10.1111/j.1464-5491.2007.02157.x.
  16. West IC. Radicles & oxidative stress in diabetes. Diabet Med 2000;17(3):171–180. DOI: 10.1046/j.1464-5491.2000.00259.x.
  17. Ramakrishna V, Jailkhani R. Evaluation of oxidative stress in insulin dependent DM (IIDM) patients. Diagn Pathol 2007;2(1):22. DOI: 10.1186/1746-1596-2-22.
  18. Wan GH, Tsai SC, Chiu DT. Decreased blood activity of G6PD associated with increased risk of diabetes mellitus. Endocrine 2002;19(2):191–195. DOI: 10.1385/ENDO:19:2:191.
  19. Zhang Z, Apse K, Pang J. High glucose inhibits G6PD via CAMP in aortic endothelial cells. J Biol Chem 2002;42(7):275–400.
  20. Katalinic V, Salamunic I, Pazanin S, et al. The antioxidant power and lipid peroxidation products in the sera of apparently healthy adult males. Coll Antrpol 2007;1(1):165–171.
  21. Varley's Practical Clinical Biochemistry. 6th ed, London: Heinemann Professional Publishing Ltd.
  22. Mohan V, Mathur P, Deepa R, et al. Urban rural differences in prevalence of self reported diabetes in India–the WHO-ICMR Indian NCD risk factor surveillance. Diabetes Res Clin Pract 2008;80(1): 159–168. DOI: 10.1016/j.diabres.2007.11.018.
  23. Sarwar N, Gao P, Seshasai SR, et al. Diabetes mellitus, fasting blood glucose concentration, and risk of vascular disease: a collaborative meta-analysis of 102 prospective studies. Lancet 2010;375(9733):2215–2222. DOI: 10.1016/S0140-6736(10)60484-9.
  24. Patel V, Kanani D, Chauhan K, et al. Assessment of oxidative stress in type 2 diabetes mellitus by measurement of plasma lipid hydroperoxides and total antioxidant capacity. Int J Adv Res 2015;3(5):1078–1084.
  25. Joshi SR, Joshi, Patel, et al. High prevalence of G6PD deficiency in Vataliya Prajapati community in Western India. Haematology 2001;31(1):57–60. DOI: 10.1163/15685590151092715.
  26. European Medical Alliance >> Haemolytic Anaemia. Defence against oxidative stress the production of reducing power. Last Updated on Wed, 08 Apr 2015.
  27. Zhang Z, Yang Z, Zhu B, et al. Increasing glucose 6-phosphate dehydrogenase activity restores redox balance in vascular endothelial cells exposed to high glucose. PLoS ONE 2012;7(11):e49128. DOI: 10.1371/journal.pone.0049128.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.