Indian Journal of Medical Biochemistry

Register      Login

VOLUME 21 , ISSUE 2 ( July-December, 2017 ) > List of Articles


Generation of Monospecific Polyclonal Antibodies to Recombinant Filarial Antigen rWbL2 and Evaluation of Its Immunodiagnostic Utility in Filariasis

Mahendra B Gandhe, Swapnali M Gandhe

Citation Information : Gandhe MB, Gandhe SM. Generation of Monospecific Polyclonal Antibodies to Recombinant Filarial Antigen rWbL2 and Evaluation of Its Immunodiagnostic Utility in Filariasis. Indian J Med Biochem 2017; 21 (2):117-123.

DOI: 10.5005/jp-journals-10054-0033

License: CC BY 3.0

Published Online: 01-12-2017

Copyright Statement:  Copyright © 2017; The Author(s).



Lymphatic filariasis is a mosquito-borne disease affecting nearly 120 million people across the world. Filarial antigen detection is a good indicator for mapping new filarial cases and for evaluation of filarial elimination programs as compared with the low sensitivity associated with the direct evidence of microfilaria (Mf) in blood samples. To overcome low sensitivity and night-time blood collection method for parasite detection in filariasis cases, the sandwich enzyme-linked immunosorbent assay (ELISA) was standardized for detection of circulating filarial antigen using monospecific polyclonal antibodies raised against recombinant filarial antigen rWbL2. In the present study, the specific antibodies raised against novel recombinant antigens rWbL2 were explored to develop suitable filarial antigen assays. It was possible to come out with a filarial antigen assay that could detect WbL2 or its equivalent antigen with 40% sensitivity (by using mouse anti-WbL2 antibody as capturing antibody), 60% sensitivity (using FSIgG human filarial serum immunoglobulin G as capturing antibody), and 100% specificity. These assays show promise to detect and monitor active filarial infection and thus prove to have potential as a useful diagnostic and monitoring tool in the elimination program.

How to cite this article

Gandhe MB, Gandhe SM. Generation of Monospecific Polyclonal Antibodies to Recombinant Filarial Antigen rWbL2 and Evaluation of Its Immunodiagnostic Utility in Filariasis. Indian J Med Biochem 2017;21(2):117-123.

  1. Prospects of eliminating lymphatic filariasis in India. ICMR Bull 2002 May-Jun;32(5-6):1-14.
  2. Are we nearly there yet? Coverage and compliance of mass drug administration for lymphatic filariasis elimination. Trans R Soc Trop Med Hyg 2015 Mar;109(3):173-174.
  3. Reaching endpoints for lymphatic filariasis elimination- results from mass drug administration and nocturnal blood surveys, South Gujarat, India. PLoS Negl Trop Dis 2017 Apr;11(4):e0005476.
  4. Mathematical modelling of lymphatic filariasis elimination programmes in India: required duration of mass drug administration and post-treatment level of infection indicators. Parasit Vectors 2016 Sep;9:501.
  5. Eliminate Filariasis: Attack poverty — the global alliance lymphatic filariasis. Proceedings of the first meeting, 4-5 May 2000. Spain: WHO; 2000.
  6. Defining the roles of vector control and xenomonitoring in the global programme to eliminate lymphatic filariasis. Report of the Informal Consultation held at WHO/HQ, Geneva, 29-31 January 2002. Document WHO/CDS/CPE/PVC/2002. Geneva: WHO; 2002.
  7. The global programme to eliminate lymphatic filariasis. Trop Med Int Health 2000 Sep;5(9):591-594.
  8. Prospects of elimination of lymphatic filariasis in India. ICMR Bull 2002;32(5-6):41-54.
  9. A simple inexpensive dot blot assay using a 66 kDa B. malayi microfilarial antigen protein for diagnosis of filarial infection in an endemic area. Trans R Soc Trop Med Hyg 2001 Mar-Apr;95(2):168-169.
  10. Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis — a multicenter trial. Filaria J 2004 Sep;3(1):9.
  11. An SXP/RAL2 protein produced by the subventral pharyngeal glands in the plant parasitic root-knot nematode Meloidogyne incognita. Parasitol Res 2005 Jan;95(1):50-54.
  12. ; Fritsch, EF.; Maniatis, T. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1996.
  13. ; Raykundalia, C. ELISA and releated enzyme immunoassays. In: Catty D, editor. Antibodies Volume II: a practical approach. New York (NY): IRL Press at Oxford University Press; 1989.
  14. Comparative evaluation of microtitre plate peroxidase and stick penicillinase enzyme immunoassays for detection of filarial antibodies using Brugia malayi Microfiflarial Excretory-Secretory Antigen. J Parasitic Dis 1996;20:173-176.
  15. Detection of circulating antigen in bancroftian filariasis by sandwich ELISA using filarial sera IgG. J Helminthol 1984 Sep;58(3):259-262.
  16. CD4+-dependent immunity to Onchocerca volvulus third stage larvae in humans and the mouse vaccination model: common ground and distinctions. Int J Parasitol 2003 Sep;33(11):1161-1171.
  17. Relationships between IgE/IgG4 epitopes, structure and function in Anisakis simplex Ani s 5, a member of the SXP/RAL-2 protein family. PLoS Negl Trop Dis 2014 Mar;8(3):e2735.
  18. A monoclonal antibody Bm Ab120 to 120 kDa B malayi antigen with diagnostic potential in Bancroftian filariasis. J Parasit Dis 2004 Jun;28(1):29-36.
  19. Immunoradiometric assay for detection of filarial antigens in human serum. J Immunol 1984 Oct;133(4):2237-2242.
  20. Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis. J Immunoassay 1990;11(4):429-444.
  21. The ICT filariasis test; A rapid format antigen test for diagnosis of bancroftian filariasis. Parasitol Today 1997 Oct;13(10):401-404.
  22. A highly specific and sensitive monoclonal antibody based ELISA for the detection of circulating antigen in bancroftian filariasis. Trop Med Parasitol 1990 Dec;41(4):403-406.
  23. Field trial of the ICT filariasis for diagnosis of Wuchereria bancrofti infections in an endemic population of Thailand. Southeast Asian J Trop Med Public Health 1999 Sep;30(3):562-568.
  24. Assessment of immunochromatographic test for rapid lymphatic filariasis diagnosis. Parasite 1999 Dec;6(4):355-358.
  25. Detection and monitoring of microfilarial ES antigen levels by inhibition ELISA during DEC therapy. Indian J Med Res 1984 Feb;79:194-198.
  26. Development of antigen detection ELISA for the diagnosis of Brugian filariasis and Bancroftian filariasis using antibodies to Recombinant filarial antigens BmSXP-1 and WbSXP-1. Microbiol Immunol 2002 Feb;46(5):327-332.
  27. The Wuchereria bancrofti orthologue of Brugia malayi SXP-1 and the diagnosis of bancroftian filariasis. Mol Biochem Parasitol 2000 Mar;107(1):71-80.
  28. Clearance of circulating filarial antigen as a measure of microfilaricidal activity of diethylcarbamazine in Wuchereria bancrofti infection. J Infect Dis 1995 Sep;172:521-526.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.