Indian journal of Medical Biochemistry

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VOLUME 21 , ISSUE 2 ( July-December, 2017 ) > List of Articles

ORIGINAL ARTICLE

Ultraviolet Absorption Spectral Scan and Fluorescence Emission Spectral Scan Analysis: Potential Tests with Diagnostic Utility in Porphyria

Archana Nimesh, Vibhuti Agarwal, Seema Garg

Citation Information : Nimesh A, Agarwal V, Garg S. Ultraviolet Absorption Spectral Scan and Fluorescence Emission Spectral Scan Analysis: Potential Tests with Diagnostic Utility in Porphyria. Indian J Med Biochem 2017; 21 (2):136-141.

DOI: 10.5005/jp-journals-10054-0037

License: CC BY 3.0

Published Online: 00-12-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

Symptoms of porphyria usually overlap with other clinical conditions, thereby leading to misdiagnosis and inappropriate treatment, especially when patient presents with uncommon features. An accurate diagnosis of porphyria can be made only when enzyme defects can be detected, which is costly and not easily available. Classically, Ehrlich's test used to screen porphyria has certain disadvantages too. Hence, we planned this study.

Aim

To develop comparatively simpler diagnostic tests feasible at tertiary care centers to work up porphyria cases.

Materials and methods

A suspected case of porphyria was screened using Ehrlich's test. Thereafter, patient's urine and serum samples were analyzed by ultraviolet (UV) absorption spectral scan and fluorescence emission spectral scan using multimode microplate reader and compared against normal controls to validate the results.

Results

The UV absorption spectral scan revealed a small peak at 410 nm for patient's urine sample, which intensified on acidification. The UV absorption spectral scan for patient's serum also showed absorbance peak at 405 nm vs normal serum. The fluorescence emission spectral scan of urine and serum of patient revealed a sharp emission peak at 626 nm, which was not present in respective samples of control. Fluorescence emission in patient's urine sample was 10.5 times more vs normal urine.

Conclusion and clinical significance

Novel methods like UV absorption spectral scan and fluorescence emission spectral scan using patient's urine and serum samples can be developed as diagnostic tests considering their practicality and affordability. Further, an algorithm formulated based on clinical features and basic lab tests can also identify the type of porphyria.

How to cite this article

Nimesh A, Agarwal V, Garg S, Mehndiratta M. Ultraviolet Absorption Spectral Scan and Fluorescence Emission Spectral Scan Analysis: Potential Tests with Diagnostic Utility in Porphyria. Indian J Med Biochem 2017;21(2):136-141.


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