Indian Journal of Medical Biochemistry

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VOLUME 24 , ISSUE 3 ( September-December, 2020 ) > List of Articles

Original Article

Study of Serum Homocysteine Level Variations in Sickle Cell Disease: A Meta-analysis Study

Chandrakant G Kamble, Prashant Hisalkar, Shekhar B Padhyegurjar, Jagdish D Powar, Bhausaheb V Jagdale

Citation Information : Kamble CG, Hisalkar P, Padhyegurjar SB, Powar JD, Jagdale BV. Study of Serum Homocysteine Level Variations in Sickle Cell Disease: A Meta-analysis Study. Indian J Med Biochem 2020; 24 (3):104-114.

DOI: 10.5005/jp-journals-10054-0166

License: CC BY-NC 4.0

Published Online: 01-12-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Background: Serum homocysteine levels have been observed to be increased in sickle cell disease (SCD). The biological mechanism of synthesis and regulation of the homocysteine remains unclear. This meta-analysis aims to provide an overview of the serum homocysteine level changes and to discuss its significance in SCD. Materials and methods: This meta-analysis is to determine serum homocysteine level changes during SCD and was conducted under the PRISMA guidelines. Without language restrictions, the articles were identified through BioMed, Embase, Pub Med® (U.S. National Library of Medicine, USA), and www.Blood, PLoS ONE, Web of Science, LILACS (Latin American and Caribbean Health Sciences Literature), ISI Web of Science, Chemical abstract services-(CAS), Europe PMC, Bio sis Previews, Elsevier Properties S.A., USA. The studies have been considered which were published before March 31, 2018. Results: Thirty comparative studies were identified out of 8,630 articles and included in the meta-analysis. From the final selected articles, the mean, standard deviation, sample size of cases vs controls were showing compared and calculated by the SPSS latest software version-24. From 30, 18 articles were statistically highly significant, and the remaining 12 articles were statistically not significant. The p <0.05 was considered to be statistically significant. The combined estimation of the meta-analysis study is highly significant, since p <0.000000, calculated “t” is 25.07, and degree of freedom is 2,829. Conclusion: The study concludes that there is an extremely significant difference between mean serum homocysteine levels in cases and healthy controls. So, the serum homocysteine level can be used as a clinical biomarker for the diagnosis of SCD.

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