Citation Information :
Chattopadhyay DK. Ratio of Serum Superoxide Dismutase and Whole Blood Glutathione Peroxidase: A Noteworthy Parameter for Tuberculosis Diagnosis. Indian J Med Biochem 2021; 25 (3):100-104.
Aim and background:Mycobacterium tuberculosis secretes extracellularly abundant amount of two proteins superoxide dismutase (SOD) and glutamine synthetase (GS) having no leader sequences. The pathogenesis of M. tuberculosis is contributed by the secretion of iron-cofactored SOD which scavenges reactive oxygen intermediates (ROI) by dismutation reaction and also blocks activation of NF-kB and mononuclear cell apoptosis. The obligatory aerobe catalase-positive M. tuberculosis also secretes selenoenzyme glutathione peroxidase (GPx) catalyzing reduction of peroxides produced by dismutation reaction at the expense of reduced glutathione. In this study, the author has computed the statistical ratio of serum SOD and whole blood GPx level and mulled to use that as a diagnostic marker for tuberculosis (TB) and to monitor the effectiveness of drug therapy.
Materials and methods: The participants were divided into three groups: Normal control; 2-Lung disease control and 3-TB patients (3A-pulmonary and 3B-extrapulmonary). The serum SOD and whole blood GPx activity were measured spectrophotometrically for all participants initially. Both of these parameters were assayed again after 1 month's usual additional treatment for groups II and III.
Results: The ratio as calculated in TB patients is >9 and 8 times, respectively, than those of normal and lung disease control subjects. With anti-TB drug therapy for 1 month, there was a significant decrease in the ratio.
Conclusion: The higher magnitude of the ratio might be well utilized to diagnose TB, the serial measurement of the said ratio during the course of A-TB drug treatment might confer effectiveness of drug therapy and diagnose drug-resistant cases.
World Health Organization. Global Tuberculosis Report. Geneva: World Health Organization; 2020. Licence CCBY-NC-SA3.01GO.
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