An increase in the incidence of chronic kidney disease (CKD) is seen worldwide due to increased burden of noncommunicable diseases and ageing. There is accumulation of several waste products in these patients due to kidney impairment, leading to various metabolic complications. Thus, for survival they have to undergo repeated hemodialysis. Therefore, the levels of urea and creatinine have to be continuously monitored in blood. Saliva as a sample for estimation of these parameters is emerging nowadays. The present study is an attempt to check the efficacy of urea and creatinine levels in saliva for assessment of CKD patients in comparison to their levels in the blood. The findings indicated urea and creatinine levels were differed significantly between CKD patients and controls in blood as well as in saliva. We also reported the significant positive correlation of salivary creatinine and urea with serum creatinine and urea, respectively. By means of receiver-operating characteristic (ROC) curve analysis, we found the cut-off value of 0.8 mg/dL and 40 mg/dL for salivary creatinine and blood urea, respectively, at highest sensitivity and specificity. So, the conclusion can be made that, salivary creatinine and urea can be used as an alternative to serum creatinine and urea values in calculating eGFR and staging of CKD. Also, collection of saliva, a noninvasive method, could be an alternative to blood, an invasive method, for diagnosis and monitoring patients with CKD.